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celltrace violet (ctv) dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher celltrace violet (ctv) dye
    Celltrace Violet (Ctv) Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+violet+ctv+dye/celltrace+violet/pmc11883473-118-5-9
    Average 90 stars, based on 1 article reviews
    celltrace violet (ctv) dye - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Activated T cell therapy targeting glioblastoma cancer stem cells
    Article Snippet: .. Collected cancer cells were stained as target cells with 2.5 μM of CellTrace Violet (CTV) dye (ThermoFisher Scientific, C34557) for flow cytometry analysis. ..

    Article Title: Activated T cell therapy targeting glioblastoma cancer stem cells
    Article Snippet: .. Collected PHA-blasts were stained as target cells with 2.5 μM of CellTrace Violet (CTV) dye (ThermoFisher Scientific, C34557) for flow cytometry analysis. ..

    Flow Cytometry:

    Article Title: Activated T cell therapy targeting glioblastoma cancer stem cells
    Article Snippet: .. Collected cancer cells were stained as target cells with 2.5 μM of CellTrace Violet (CTV) dye (ThermoFisher Scientific, C34557) for flow cytometry analysis. ..

    Article Title: Activated T cell therapy targeting glioblastoma cancer stem cells
    Article Snippet: .. Collected PHA-blasts were stained as target cells with 2.5 μM of CellTrace Violet (CTV) dye (ThermoFisher Scientific, C34557) for flow cytometry analysis. ..

    Labeling:

    Article Title: Signaling through the inhibitory Fc receptor FcγRIIB induces CD8 T cell apoptosis to limit T cell immunity
    Article Snippet: .. Where indicated, OT-I T cells were labeled with 5uM CellTrace Violet (CTV) dye (Life Technologies, Invitrogen) according to manufacturer’s instructions and then adoptively transferred into naïve hosts. .. Proliferation was measured on day 14 post transplantation via flow cytometry on a BS LSR II (BD Biosciences) and data were analyzed with FlowJo (Tree Star, San Carlos, CA) and Prism (GraphPad Software).

    Article Title: Smooth Muscle Cell–Derived Interleukin-17C Plays an Atherogenic Role via the Recruitment of Proinflammatory Interleukin-17A + T Cells to the Aorta
    Article Snippet: Erythrocytes were lysed using ACK lysis buffer (8.29mg/ml NH4Cl, 1mg/ml KHCO3, 0.372 mg/ml EDTA, pH 7.2, all from Sigma Aldrich). .. Leukocytes were subsequently labeled with 5μM CellTrace Violet (CTV) dye (Molecular Probes, Life Technologies) in PBS at 37C for 10 minutes and washed twice with PBS containing 1% FBS. ..

    Article Title: Ferroptosis Suppressor Protein 1 Inhibition Promotes Tumor Ferroptosis and Anti-tumor Immune Responses in Liver Cancer
    Article Snippet: .. We first pre-treated mouse HCC cells Hepa1-6 with iFSP1 or vehicle control and labeled them with CellTrace Violet (CTV) dye (Invitrogen). ..

    Control:

    Article Title: Ferroptosis Suppressor Protein 1 Inhibition Promotes Tumor Ferroptosis and Anti-tumor Immune Responses in Liver Cancer
    Article Snippet: .. We first pre-treated mouse HCC cells Hepa1-6 with iFSP1 or vehicle control and labeled them with CellTrace Violet (CTV) dye (Invitrogen). ..



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    Knockdown of ZC3H11A by ASO induces immunogenic apoptosis in B16 melanoma cells (A) The percentage of calreticulin (CRT) + cells analyzed out of viable cells by flow cytometry in B16 cells after 36 h of ASO transfection. (B) ATP levels (RLU) in the supernatant of ASO-transfected B16 cells. (C and D) The percentage of dendritic cells (DCs) that phagocytized ASOs transfected with B16 stained with <t>CellTrace</t> violet dye (CTV) and representative plots of flow cytometry. Mean fluorescence intensity (MFI) of DC activation and maturation markers (E) CD40, (F) CD86, and (G) MHC class II after co-culture of DCs with ASO-transfected B16 cells. (H–J) Representative histograms of the activation markers. n = 3 replicates/group for (A)–(C) and (E)–(G). Error bars represent SDs, and the mean values were compared using an unpaired two-tailed t test. ns, non-significant; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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    Thermo Fisher ctv-labelled (celltrace violet cell proliferation dye
    Knockdown of ZC3H11A by ASO induces immunogenic apoptosis in B16 melanoma cells (A) The percentage of calreticulin (CRT) + cells analyzed out of viable cells by flow cytometry in B16 cells after 36 h of ASO transfection. (B) ATP levels (RLU) in the supernatant of ASO-transfected B16 cells. (C and D) The percentage of dendritic cells (DCs) that phagocytized ASOs transfected with B16 stained with <t>CellTrace</t> violet dye (CTV) and representative plots of flow cytometry. Mean fluorescence intensity (MFI) of DC activation and maturation markers (E) CD40, (F) CD86, and (G) MHC class II after co-culture of DCs with ASO-transfected B16 cells. (H–J) Representative histograms of the activation markers. n = 3 replicates/group for (A)–(C) and (E)–(G). Error bars represent SDs, and the mean values were compared using an unpaired two-tailed t test. ns, non-significant; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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    Image Search Results


    Knockdown of ZC3H11A by ASO induces immunogenic apoptosis in B16 melanoma cells (A) The percentage of calreticulin (CRT) + cells analyzed out of viable cells by flow cytometry in B16 cells after 36 h of ASO transfection. (B) ATP levels (RLU) in the supernatant of ASO-transfected B16 cells. (C and D) The percentage of dendritic cells (DCs) that phagocytized ASOs transfected with B16 stained with CellTrace violet dye (CTV) and representative plots of flow cytometry. Mean fluorescence intensity (MFI) of DC activation and maturation markers (E) CD40, (F) CD86, and (G) MHC class II after co-culture of DCs with ASO-transfected B16 cells. (H–J) Representative histograms of the activation markers. n = 3 replicates/group for (A)–(C) and (E)–(G). Error bars represent SDs, and the mean values were compared using an unpaired two-tailed t test. ns, non-significant; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Targeting ZC3H11A elicits immunogenic cancer cell death through augmentation of antigen presentation and interferon response

    doi: 10.1016/j.omtn.2024.102361

    Figure Lengend Snippet: Knockdown of ZC3H11A by ASO induces immunogenic apoptosis in B16 melanoma cells (A) The percentage of calreticulin (CRT) + cells analyzed out of viable cells by flow cytometry in B16 cells after 36 h of ASO transfection. (B) ATP levels (RLU) in the supernatant of ASO-transfected B16 cells. (C and D) The percentage of dendritic cells (DCs) that phagocytized ASOs transfected with B16 stained with CellTrace violet dye (CTV) and representative plots of flow cytometry. Mean fluorescence intensity (MFI) of DC activation and maturation markers (E) CD40, (F) CD86, and (G) MHC class II after co-culture of DCs with ASO-transfected B16 cells. (H–J) Representative histograms of the activation markers. n = 3 replicates/group for (A)–(C) and (E)–(G). Error bars represent SDs, and the mean values were compared using an unpaired two-tailed t test. ns, non-significant; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Article Snippet: For DC phagocytosis, ASO-treated cells were stained with CellTrace violet dye (CTV, 5 μg/mL) (Thermo Fisher Scientific) and co-cultured with imDCs for 2 h. The CTV + DCs were considered phagocytic cells and were quantified by flow cytometry after gating for CD11c + (phycoerythrin [PE] anti-mouse CD11c [BioLegend]).

    Techniques: Knockdown, Flow Cytometry, Transfection, Staining, Fluorescence, Activation Assay, Co-Culture Assay, Two Tailed Test